Biology/nutrient agar
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Question
halo sir/mam,
I'm doing my project in marine with copper resistant bacterial study.for that purpose i need to prepare nutrient agar in sea water that is sterilized prior using filter as told by my guide.but my question is, once i prepare agar slants,am able see granule particles in the test tube between the media in test tube.i want to know what is the reason for that granules formation? is it because of the salt that has precipitated which is found in the sea water?and how can i avoid that even though i perform in highly sterilized condition...I'm helpless kindly help me...
Hi Meenakshi: Thanks for your question. I think that what you are seeing are precipitated salt crystals, perhaps calcium salts which are less soluble than sodium or potassium salts. I don't think this will be a problem. The bacteria will still be able to access the salts as needed.
Hope this helps.
FM Rollwagen, PhD
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Answers by Expert:
Florence M Rollwagen
Expertise
I can answer questions in biology, microbiology and immunology on the undergraduate or graduate level. I can also address medical and health concerns regarding alternative medicine, autoimmune diseases (lupus, MS) liver disease and intestinal problems.
Experience
I have over 20 years experience in research and teaching at the medical/graduate level, and 5 years teaching college biology and microbiology. My expertise is in microbiology and immunology, specifically the biology of cytokines and soluble immune response modifiers. I also carried out original research in blood substitutes and shock/trauma.
Organizations
American Association of Immunologists (AAI)
American Association for the Advancement of Science (AAAS)
Publications
Journal of Experimental Medicine, Journal of Immunology, Cytokine, Shock, Experimental Hematology
Education/Credentials
BS biology 1966
MS biology 1968
PhD immunology 1979
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