Biology/Microbiology
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Question
What would happen if you underdecolorize a gram positive organism? And the same question for gram negative organism?
Hi Gillian: Thanks for your question.
If, by "undercolorize" you mean you didn't leave the crystal violet on long enough, you would get a light blue gram positive organism. For a gram negative, the counterstain is red, so you would have light blue and pink organisms.
Is this what you wanted to know? If not, write back and we'll discuss it some more.
FM Rollwagen, PhD
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Answers by Expert:
Florence M Rollwagen
Expertise
I can answer questions in biology, microbiology and immunology on the undergraduate or graduate level. I can also address medical and health concerns regarding alternative medicine, autoimmune diseases (lupus, MS) liver disease and intestinal problems.
Experience
I have over 20 years experience in research and teaching at the medical/graduate level, and 5 years teaching college biology and microbiology. My expertise is in microbiology and immunology, specifically the biology of cytokines and soluble immune response modifiers. I also carried out original research in blood substitutes and shock/trauma.
Organizations
American Association of Immunologists (AAI)
American Association for the Advancement of Science (AAAS)
Publications
Journal of Experimental Medicine, Journal of Immunology, Cytokine, Shock, Experimental Hematology
Education/Credentials
BS biology 1966
MS biology 1968
PhD immunology 1979
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