AboutParas Anand Expertise I have studied molecular biology & cell biology. I would be happy to answer your general questions on cells, their organelles, membranes and host-pathogen interaction. I will not answer questions on basic biology.
Question Hypothetical question. If I am studying a protein which is localized to the center of the cell, when I treat it with a growth factor. I want to prove that the protein is entering the nucleus. What kind of imaging will show the protein is in the nucleus not just above or below it in the cytoplasm.
Answer You have to make z-section of the fixed cells in which nucleus is stained with DAPI. Labeling your protein with fluorescent antibody and looking for co-localization with the nucleus can tell if the protein indeed enters the nucleus.
