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Chemistry (including Biochemistry)/Beer's law
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Question
What are some available methods that could be used
to deproteinise serum samples prior to analysis?
I got two sets of absorbance values for standards in the detection of serum phosphorus... I just don't understand why the absolute absorbance values differ detween the two instruments (plate reader and the spectrophotometer). Can u tell me why?
Spectrophotometer : 620 nm wavelenght
Plate reader: 650 nm wavelength
Which term in the context of beer's law that accounts for the difference in absorbance between the same solutions read on the spectrophotometer and the plate reader?
Beers law is A=abc, but if you write it out completely it is
A (at wavelength lamda) = a (at wavelength lamda) bc
It is wavelength dependent, which it must be. If not you would be saying that a solution absorbs light the same at every wavelength. That would make a boring world since ther would be no color. Everything would be white.
http://www.chemistry.adelaide.edu.au/external/soc-rel/content/beerslaw.htm
http://www.ic.sunysb.edu/Class/che133/lectures/beerslaw.html
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Answers by Expert:
Dr. Henry Boyter, Jr.
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Experience in the area
Chemistry (non-biochemistry), environmental science, occupational health and safety, environmental regulation and management, environmental engineering, and wastewater engineering. I'm the Director of Environmental, Health, and Safety and the Director of Research at the Institute of Textile Technology.
Experience
Chemistry (non-biochemistry), environmental science, occupational health and safety, environmental regulation and management, environmental engineering, and wastewater engineering. I'm the Director of Environmental, Health, and Safety and the Director of Research at the Institute of Textile Technology.
Education/Credentials
PhD, MS, BS in Chemistry
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