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Genetics/Cloning

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Question
Hi,

I have a question about cloning. When wanting to express a gene in a certain cell line a plasmid containing multiple copies of that gene can be used (3-6 copies). My question is simply why this is done?

Answer
hi Ana,

One reason is to increase the yield of the protein product of that gene. Some genes may have poor expression or may have poor transfection efficiency in some cell lines. For this reason, increasing the copy number of the gene in the plasmid, might help to increase the expression levels of the gene and its protein product.

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Thanemozhi Natarajan

Expertise

Answers in Genetics, genomics, cytogenetics of syndromes, congenital anomalies, cancer, clinical genomics and interpretation of omics data.

Experience

More than 10 years. Doctoral research thesis on Congenital anomalies and cytogenetics, Recurrent reproductive failure and chromosomal abnormalities. Postdoctoral experience in Breast cancer research. Current: Clinical Genomics and Pharmacogenomics.

Organizations
Academic

Publications
Cancer Cell International, Cancer Epidemiology Biomarkers and Prevention, Breast cancer research and treatment, Indian Journal of Pediatrics, BMC Proceedings, Pharmacogenetics and Genomics, Human Molecular Genetics, Frontiers in Genetics, Cancer Biomarkers.

Education/Credentials
PhD Biomedical Genetics

Awards and Honors
University Grants Commission Award for pursuing PhD level research (India); Travel awards to attend conferences.

Past/Present Clients
Post doctoral experience Cancer research, molecular epidemiology Current: Clininical Genomics and Pharmacogenomics

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