About Shane Stecklein Expertise I can answer general questions in molecular and statistical genetics as well as those relating to general cellular and molecular biology. My primary interests are in cancer biology and immunology.
Experience I am currently an undergraduate pre-medical student pursuing a B.S. in cellular biology with a minor in chemistry. I work as a research assistant in a molecular biology laboratory investigating the Adenomatous Polyposis Coli (APC) gene--this locus is mutated in over 80% of all colorectal cancers--my individual project is to investigate the interaction of the APC protein with elements of the cytoskeleton (particularly cytokeratin 18) in colonic epithelial cells. Our primary goal is to discern the mechanism by which the nuclear function (or lack thereof) of the mutated protein results in tumorgenic polyps. I also teach undergraduate laboratory courses in chemistry and microbiology at the University of Kansas. Upon completion of my undergraduate degree, I hope to enter an M.D., Ph.D. progam and pursue a career in radiology and nuclear medicine and continue to do cancer research.
Organizations Tri-beta Honors Society
American Society for Microbiology
National Society of Collegiate Scholars
American Chemical Society
Education/Credentials B.S. - Cellular Biology /w minor in Chemistry (Honors) (in progress)
Awards and Honors State of Kansas Scholar
University of Kansas Honor Scholar
Mount Oread Scholar
National Collegiate Scholar
Question This is a problem that I was given in my biology class to set up a trihybrid punnett square, and I don't know how to set the cross up, I've typed the question as it appears on the worksheet.
-->You are a world-famous breeder of Purple-People Eaters ("PPE"). Tragically, your entire stock perishes in a fire except for two individuals. They are both heterozygous for all three traits desired. The three traits are as follows:
The traits that bring the big bucks ($100 each) are "one eyed, one horned, flying" purple people eaters. If you breed the remaining two PPE, what distributions of traits will you get?
Of these, how many will have all three of the traits that you will be able to sell for those big bucks?
It costs $25 to raise a PPE. Will it be cost effective (get a lot of them critters) or shall you buy yourself fresh breeding stock of a different genotype? Briefly explain.
THAT IS THE QUESTION THAT I AM HAVING A PROBLEM SOLVING, YOUR HELP WOULD BE GREATLY APPRECIATED TO HELP KEEP ME FROM FAILING MY COURSE
Answer Hi Dee--
Regarding your statistical genetics question:
Since you are dealing with a tri-hybrid cross, you can assume that there will be 64 individuals created due to unique generation of gametes (2^6 (or 2(2^3))). If you were to cross two individuals of genotype EeHhFf, you would get the following distributions:
Two Eyed, Two Horned, Non-Flying.....27 .....42.1%
Two Eyed, Two Horned, Flying.........9 .....14.1%
Two Eyed, One Horned, Non-Flying.....9 .....14.1%
Two Eyed, One Horned, Flying.........3 ......4.7%
One Eyed, Two Horned, Non-Flying.....9 .....14.1%
One Eyed, Two Horned, Flying.........3 ......4.7%
One Eyed, One Horned, Non-Flying.....3 ......4.7%
One Eyed, One Horned, Flying.........1 ......1.5%
So, out of those, you will only be able to sell 1 (or 1.5% of all the offspring for "the big bucks". If it costs $25 to raise a PPE, then it definately IS NOT going to be cost effective to generate your own stock of eehhff organisms... To statistically generate a breeding pair, you would have to get 128 offspring (which would account to $3,200). So, if you can buy two eehhff PPEs for less than this amount, it would be much more cost effective to do so...
