Molecular Biology/Questions Answered by Expert Andrew Leung
| Subject | Date Asked |
| question | 12/30/2006 |
| Q: my question is: is segregation importan in asexual reproduction, and if it is why is it or if it ... A: Segregation is important in both sexual and asexual reproduction. It is important in the sense that ... | |
| molecular biology | 12/25/2006 |
| Q: i'm from india. One day in my dream i found one experiment sir, that is during amplification of ... A: your dream indeed gave you and others a very good idea. If what you have described can be realised, ... | |
| molecular biology | 12/19/2006 |
| Q: what is the role of internal control in realtime pcr A: Just like all other experiments or tests, a control serves to make sure that everything is working ... | |
| molecular biology of hepatitis b virus | 12/18/2006 |
| Q: how the classification of hepatitis b virus was done and on what basis?and what are the ... A: Many classification of hepatitis B virus exist. There are several genotypes of HBV, based on ... | |
| restriction site mapping | 12/8/2006 |
| Q: i dont understand what is restriction site mapping! iv done an experiment on this where we had to ... A: Restriction site mapping is about locating "restriction site", a known short sequence recognizable ... | |
| evolutions | 12/5/2006 |
| Q: As cells evolve, over long periods of time, to more complex life forms, do bacterias and viruses, ... A: I can't say you are wrong because most of the facts you mentioned are valid. However, you may have a ... | |
| SDS-PAGE band clarity | 11/30/2006 |
| Q: I've been running loads of SDS-PAGE samples lately, but now have a problem. One sample submitter ... A: It is likely that the fuzzy appearance is due to lack of reducing agent. Please bear in mind that ... | |
| Microscope | 11/29/2006 |
| Q: I am an earth science student looking for a bi-focal microscope around $200 that is not a toy. It ... A: It is hard to comment because I don't have data to compare. However, based on appearance, they look ... | |
| Latin words in biology | 11/21/2006 |
| Q: Why does biology, physics, and other sciences use so many latin words? Why not use strickly English ... A: People uses Latin words because Latin is very old language. It was widely used in many old books. ... | |
| Question about Ethidium Bromide | 10/28/2006 |
| Q: Andrew, how to discard/neutralise ethidium bromide after used for DNA staining? A: Charcoal will absorb ethidium bromide. I knew some labs will put a pack of charcoal into the tank ... | |
| extracellular ATP | 10/3/2006 |
| Q: can you please tell me can mammalian cells utilize extracellular ATP as an energy source. Thank you ... A: I am unsure if mammalian cells can utilize extracellular ATP. However, I think extracellular ATP may ... | |
| mostly genetics, maybe a bit molecular bio | 10/1/2006 |
| Q: I read in the text that proteins are sometimes made of multiple polypeptide chains (ADH or alcohol ... A: If heterozygote, four protein species will be resulted, i.e. M/M, M/N, N/M and N/N, where M is a ... | |
| genetics & molecular biology | 9/25/2006 |
| Q: I was wondering what are a few advantages/disadvantages to having multiple chromosomes? Thanks for ... A: The advantages are obvious. For example, the ability to realise cross over, random assortment, ... | |
| DNA Computer | 9/16/2006 |
| Q: "Hello sir i want to ask u a question..about hw to convert DNA enzyme into binary code..what is the ... A: I want to request more information from you about question. It interested me and I wish to you ... | |
| E. coli. | 9/15/2006 |
| Q: Hey Andy At what pH level does E. coli. reproduce at its optimum? Is it acidic/ alkali or neutral ... A: I think this link is good reference to read: http://textbookofbacteriology.net/nutgro.html See ... | |
| genetics | 9/11/2006 |
| Q: my question is Some genes are only present in only one copy in some organisms (only one allele is ... A: In this case, you don't have to know whether it is a dominant or recessive. Strictly speaking, it is ... | |
| Anions and cations | 9/10/2006 |
| Q: Can you explain to me in simple terms Why Anions and Cations are important? A: Your question involves scope which is too wide to be answered. However, in essense, when a salt ... | |
| plasmids | 9/9/2006 |
| Q: ive got this question and its stumped me totaly, i dont even understand what its asking me Plasmids ... A: First, I asssume that you are talking about bacterial cell culture. Because, plasmid replicates in ... | |
| RESTRICTION MAP | 9/5/2006 |
| Q: the circle drawing. i have absolutly no idea how. google just brings up the actual experiment to ... A: I haven't come across any good plasmid drawing tools in the past. In reality, people seldom draw ... | |
| oligonucleotides | 8/23/2006 |
| Q: i have been given this question as part of my uni coursework and cannot find anything that has ... A: The primers are: (forward primer) 5'-GCATGTACGTCCTTG-3' (reverse primer) 3'-CAGGTTTTTTTAAAG-5' ... | |
| Problems with In Vitro Transcription | 7/26/2006 |
| Q: I have been trying to get a transcription to work for a few weeks. I believe I have exausted many ... A: If you think EB is not sufficient, use double or triple amount of EB is acceptable. However, I think ... | |
| bacterial growth on agar plates | 7/23/2006 |
| Q: can you streak bacteria onto agar plates and instead of putting them in the incubater at 37 degrees ... A: Store at 4 deg. C will almost stop growth of the bacteria, and, very likely, they are remain viable. ... | |
| Genotyping | 7/19/2006 |
| Q: I am a recent graduate of UNC-Chapel Hill and I just started working in a behavioral neuroscience ... A: I am unable to answer all your questions. However, let me try some of them. Also, I may wrong! 1) ... | |
| heating water | 7/18/2006 |
| Q: I have been unable to get a answer for the following question after looking for several weeks now I ... A: 1. Statement 1 is correct. 2. Statement 2 is probably in large part correct. 3. Statement 3 is quite ... | |
| In vitro transcription | 7/12/2006 |
| Q: i am trying to trancribe 2kb fragment with T7 RNA polymerase but the product formed is shorter than ... A: 2kb is a bit longer than usual, but it should be no problem. Let me suggest a couple of questions ... | |
| random hexamers | 7/6/2006 |
| Q: I have a question about reverse transcription using random primers. I assumed that by working with ... A: I assume that you are asking me whether random hexamers will flavor amplification towards the 5' end ... | |
| Real time PCR | 6/26/2006 |
| Q: I´m a pHD student at Valencia´s university. I usually perform Real time PCR with cDNA of samples of ... A: My suggestion is that if you have been using it and it worked fine so far, it is already a good ... | |
| DNA storage | 6/12/2006 |
| Q: I would like to know for how long is it possible to storage DNA (in water) at 4oC? What are the ... A: The major enemy of DNA is the nuclease enzymes which will turn DNA into pieces. Unfortunately, ... | |
| Microbial genetics | 6/7/2006 |
| Q: Av. Molecular wt. of HUMAN DNA (n) is about 310 g/mol per nucleotide with approximate 3.2*109 (10 to ... A: You can first calculate what is the Av. Molecular Wt. of a nucleotide based on the first statement. ... | |
| biology | 5/31/2006 |
| Q: f+,Na+,C1- and HCO3 all have charges and therefore are called ionic bond is that right? A: To a certain extent you are right. Let me explain it in more detail using Na as an example. The ... | |
| antibacterial gels harmful to you? | 5/9/2006 |
| Q: I hope I'm finding you in the best of health.I saw places like Crabtree&Evelyn sell antibacterial ... A: Without having the exact formula of the product, I could not produce a good answer to your question. ... | |
| biology | 5/2/2006 |
| Q: I am homeschooling my daughter, using in part an internet program. So far I have been able to work ... A: My answers, though may not be necessarily correct, are summaried as below: Q1. (D) - Conversion of ... | |
| E.coli growth | 4/4/2006 |
| Q: plated out transformed DH5 alpha E.coli representing a cDNA library from glycerol stocks onto ... A: I think I have encountered a similiar situation before and I believe that it may have many possible ... | |
Andrew Leung
Top Expert on this page
Expertise
I`ve got a higher degree in Basic Medical Science and I can answer questions related to modern molecular biology principles, techniques and products.
Experience
Ph.D. in Basic Medical Science with post-doctoral experience
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