AboutMichael Anisfeld Expertise Disclaimer:
SORRY BUT I DO NOT ANSWER QUESTIONS RELATED TO: DRUG ACTIONS/INTERACTIONS, INTERNET DRUG PURCHASES, RESULTS OF DRUG TESTS, IDENTIFYING DRUGS (FOR WHICH YOUR LOCAL PHARMACIST IS THE BEST PERSON TO CONSULT).
My expertise is answering questions relating to pharmaceutical manufacturing and quality technologies, drug regulations and specifically GMP requirements
Experience
Past/Present Clients UN agencies (UNFPA, UNICEF, UNIDO)
Governments (Australia, Canada, India, United Kingdon, United States
Companies - over 200 companies in 37 countries
A question regarding standard concentrations within a calibration curve. If you are going to build an assay with a dynamic range of 1 to 1000 ng/mL, with 8 standards is there a systematic way of deciding where to place your standards? 1, 2, 10, 50, 100, 500, 900, 1000? Some say on a logarithmic scale but is there an easy way to calculate that?
Answer Dear Steve:
Most folks will build their calibration curve with standards at 25%, 50%, 75%, and 100% (some folks also add 125%) potency.
The key then is to check that the correlation coefficient is "> 0.98%" (some different country authorities want "> 0.99%") If you get less, then you cannot use the assay.
